LOCUS       40924_2627        4876 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector p35S-loxP-BSD DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Moss transformation vector p35S-loxP-BSD
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Aoyama T, Hiwatashi Y, Hasebe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Contact:Yuji Hiwatashi National Institute 
            for Basic Biology, Evolutionary Biology; Myodaiji, Nishigonaka, 38, 
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 4876)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2009) Contact:Yuji Hiwatashi National Institute for Basic 
            Biology, Evolutionary Biology; Myodaiji, Nishigonaka, 38, Okazaki, 
            Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     708..741
                     /label=loxP
                     /note="loxP"
     protein_bind    708..741
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        884..1229
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1268..1654
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="PLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVNV
                     YHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKAI
                     VKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    1800..1976
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(2563..2596)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(2688..2706)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2727..2743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2751..2767)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2775..2805)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2820..2841)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3129..3717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3891..4748)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4749..4853)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattccata acttcgtata
      721 gcatacatta tacgaagtta tgccccccta ctccaaaaat gtcaaagata cagtctcaga
      781 agaccaaagg gctattgaca cttttcaaca aagggatcga attcctgcag cccccctact
      841 ccaaaaatgt caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
      901 gggtaatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
      961 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
     1021 tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1081 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct
     1141 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1201 aaggaagttc atttcatttg gagaggacag cccaagcaag gaggtgagct ttctcattgt
     1261 caatatgcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     1321 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     1381 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     1441 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     1501 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     1561 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     1621 tgaattgctg ccctctggtt atgtgtggga gggctaagca ccagccattg atcttgtaca
     1681 ttcaagctac tttctgttac tagatgaacc gagcatgagt tgatggtgac gcgggactct
     1741 ggggttcgga cggtacgctg aaatcaccag tctctctcta caaatctatc tctctctatt
     1801 ttctccataa ataatgtgtg agtagtttcc cgataaggga aattagggtt cttatagggt
     1861 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     1921 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatctcct
     1981 aaagtcccta tagatctttg tcgtgaatat aaaccagaca cgagacgact aaacctggag
     2041 cccagacgcc gttcgaagct agaagtaccg cttaggcagg aggccgttag ggaaaagatg
     2101 ctaaggcagg gttggttacg ttgactcccc cgtaggtttg gtttaaatat gatgaagtgg
     2161 acggaaggaa ggaggaagac aaggaaggat aaggttgcag gccctgtgca aggtaagaag
     2221 atggaaattt gatagaggta cgctactata cttatactat acgctaaggg aatgcttgta
     2281 tttataccct atacccccta ataacccctt atcaatttaa gaaataatcc gcataagccc
     2341 ccgcttaaaa attggtatca gagccatgaa taggtctatg accaaaactc aagaggataa
     2401 aacctcacca aaatacgaaa gagttcttaa ctctaaagat aaaagatctt tcaagatcaa
     2461 aactagttcc ctcacaccgg tgacggggat cgcatgcgat cctcaccaaa atacgaaaga
     2521 gttcttaact ctaaagataa aagatctttc aagatcaaaa ctataacttc gtatagcata
     2581 cattatacga agttatccct cacaccggtg acggggatcg catgcccggg tctagacata
     2641 tggatcctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     2701 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2761 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2821 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2881 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2941 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3001 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3061 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3541 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3721 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3781 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3841 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3901 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3961 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4021 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4081 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4141 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4201 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4261 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4321 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4381 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4441 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4501 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4561 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4621 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4681 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4741 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4801 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4861 ccccgaaaag tgccac
//
